Analysis Note

Positive ControlHeLa cells

Application

ELISA (see comments)Immunoblotting (1-5 g/ml)Immunocytochemistry (10 g/ml)

General description

Purified mouse monoclonal antibody. Recognizes the HMGA2 protein.

Recognizes the HMGA2 protein in HeLa cells.

Anti-HMGA2, mouse monoclonal, clone 2D10, recognizes the HMGA2 protein in HeLa cells. It is validated for use in ELISA, Western blotting, and immunocytochemistry.

Immunogen

A recombinant polypeptide corresponding to amino acids of 1-93 human HMGA2, expressed as a GST fusion protein

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.

Physical form

In PBS, pH 7.9.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Warning

Toxicity: Regulatory Review (Z)